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PerFuseIt manifold enabling continuous media circulation for 3D cell cultures, compatible with various well plate sizes and t
Drip & Sip mode of PerFuseIt, delivering media into single wells and removing it from the top, ideal for organoids and sphero

Perfuse 3D biology: organoids, spheroids, and 3D tissues.

The PerFuseIt manifold is customizable to different well plate sizes and can provide continuous media circulation of static cultures. The system can also integrate with transwell inserts to incorporate a flow-through collection and analysis strategy for your assays.

UL-certified BioBot Basic 3D benchtop, entry-level bioprinter loaded up with five material types

Perfuse 3D Cell and Tissue Cultures

BioAssemblyBot performing precise 3D bioprinting of living cells into structured tissue models for regenerative medicine rese
3D bioprint cells
into well plate
PerFuseIt cassette designed for secure, leak-free connection to well plates and perfusion manifolds in automated 3D cell cult
Attach PerFuseIt
cassette and pumps
PerFuseIt and incubate in-vitro
Perfuse and incubate
3D model in-vitro
Overview
Drip & Sip mode of PerFuseIt, delivering media into single wells and removing it from the top, ideal for organoids and sphero

Drip & Sip.

Drips media into single wells and sips it out. Ideal for organoids and spheroids. Inlet drips media into a well and outlet sips from the top of the collected media within the well, maintaining a particular fluid volume. Input/output heights and spatial locations of the well are customizable.

Transwell.

Perfuse into basket and out of membrane. Ideal for cell interaction studies. Inlet drips media into transwell basket and media flows through cell/tissue culture and porous transwell mesh into the well below. The outlet sips from the top of the collected media to maintain a specific fluid height. Transwell provides hydrostatic pressure-driven flow through 3D tissue or cell culture. 

PerFuseIt system configured for Transwell plates, perfusing media into baskets and through porous membranes to support co-cul
PerFuseIt MicrofluidCutout component designed for precise integration with microfluidic devices to enable automated perfusion

Full Microfluidic.

Automate perfusion via BioAssemblyBots, ideal for high-throughput 3D assays. Input/output ports directly interface with the tissue construct’s vascular channels. Pump drives the media/buffer flow through the construct and out. 

Applications
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Better Tissue Conditioning. 

Flow-based conditioning of engineered tissues.

More Viable Biology.

Enable better cell & tissue viability, function and longevity with cell nourishment.

Organs-on-a-Chip.

Create in-vitro perfusable organ models easily to study microphysiological environments and test reactions.

Easier Angiogenesis Assays.

Direct vascular self-assembly with nourished stromal cells.

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Tech Specs

PerFuseIt Key Features & Specifications:

PerFuseIt system connected to a single-channel syringe pump enabling precise media perfusion for 3D tissue cultures.

Integrates with Standard Syringe Pumps

PerFuseIt manifold enabling continuous media circulation for 3D cell cultures, compatible with various well plate sizes and t

Perfuse bioprinted constructs, organoids, and spheroids

Compatible with all culture medias and reagents

Compatible with all culture medias and reagents

Microfluidic Management

Integrates directly into your workflow

* BioAssemblyBot 400 with integrated smart sensors and interchangeable tools for scalable and reproducible biofabrication.

Use with BioAssemblyBots

200, 400, & 500

Manifold Configurations Available:

6, 12, 24, 48, or 96-well plates

Compatible Well Plates:

Corning Costar 6, 12, 24, 48,

or 96-well plates

BioStorageBot Benchtop cassettes available

BioStorageBot Benchtop

cassettes available

BioApps are applicable

Compatible

Compatible Pumps:

Any single or multi-channel

syringe pump

Recommended Tubing:

0.0625” outer diameter,

0.03” inner diameter FEP tubing 

Full Microfluidic mode of PerFuseIt, automating perfusion via BioAssemblyBots for high-throughput 3D assays, interfacing dire
PerFuseIt manifold enabling continuous media circulation for 3D cell cultures, compatible with various well plate sizes and t
PerFuseIt system manufactured in a UL-certified facility meeting GMP requirements, ensuring high-quality standards

The Advanced Solutions Louisville, KY facility has been audited by UL Registrar LLC and meets GMP requirements listed in RCP, NBCP, or Pharmaceutical Certification Schemes which is uniquely accredited by the American National Standard Institute (ANSI) developed in accordance with applicable sections of the FDA’s code of federal regulations.

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