Enable your precious 3D cell culture to survive and thrive
Control Fluid & Media Delivery to Cell and Tissue Cultures
Integrate microfluidic management into your workflow
Perfuse 3D bioprinted constructs, organoids, spheroids, and assays
Easy to handle, setup & sterilize
Connects with standard syringe pumps
Compatible with all culture medias and reagents
Compatible with 96, 48, 24, & 6-well plates
Why add perfusion to 3D cell culture?
Nourishment enables better cell & tissue viability, function and longevity
Flow-based condioning of engineered tissues
Better replicates the in-vivo environment
More informative drug screening results
A Cassette for Every Experiment
Cassettes are customizable, compatible, and compact
Drip & Sip
Drips media into single wells and sips it out. Ideal for organoids and spheroids.
Transwell
Perfuse into basket and out of membrane. Ideal for cell interaction studies.
Full Microfluidic
Automate perfusion via BioAssemblyBot. Ideal for high-throughput 3D assays.
PerFuseIt Drip & Sip
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Inlet drips media into a well
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Outlet sips from the top of the collected media within the well, maintaining a particular fluid volume
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I/O heights and spatial locations of the well are customizable
PerFuseIt Transwell
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Inlet drips media into transwell basket
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Media flows through cell/tissue culture and porous transwell mesh into the well below
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Outlet sips from top of the collected media to maintain a specific fluid height
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Provides hydrostatic pressure-driven flow through the 3D tissue or cell culture
PerFuseIt Full Microfluidic
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I/O ports directly interface with the tissue construct's vascular channels
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Pump is driving the media/buffer flow through the construct and out
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Automate perfusion workflow via BioAssemblyBot
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Ideal for high-throughput 3D or 2D assays
How to Add Perfusion to 3D Cell and Tissue Culture
3D bioprint cells into well plate
Attach PerFuseIt cassette and pumps
Perfuse and incubate 3D model in-vitro
Key Applications
BioAssemblyBot and IN Cell Analyzer adds value to tissue engineering, drug discovery, and precision medicine
Build channel perfusion into 3D culture
Automated Assembly of PerFuseIt + In-Vitro Module (IPM)
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Design in TSIM and fabricate with BioAssemblyBot a perfused microvasculature system - with or without parenchyma
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Recapitulates native vascularization (angiogenesis and remodeling)
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Integrates with precision medicine workflows: assays, screens, and therapies
Build truly vascularized, living tissues
What happens when we combine microvessels, perfusion, and the perfect conditions?
PerFuseIt Specifications:
Sizes
Chips available with 6, 12, 24, 48, or 96 well size
Materials
Stainless Steel Hypotubes of desired diameter and length
Compatible Incubators
Fits within any standard incubation system
Compatible Well Plates
Corning Costar 6, 12, 24, 48, or 96 Well Plates
Compatible Pumps
Any single or mult-channel syringe pump
Recommended Tubing
1/16" OD FEP tubing for connection
Ready to perfuse your cell or 3D tissue culture?
