To begin exploring the utility of the model for NAFLD/NASH, liver tissues were exposed to a high fat medium (HFM) comprised of a base medium supplemented with glucose (25mM), palmitic acid (45μM), and sodium oleate (65μM).
ASLS has developed a vascularized, human adipose organoid (vAO) to model adipose tissue biology in vitro using a simple and robust approach involving isolated, intact microvessel fragments (haMVs).